Dear users, we have installed a new software: unblur 1.0.2:
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unblur: unblur/1.0.2
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Description:
Unblur is used to align the frames of movies recorded on an electron microscope to reduce image blurring due to beam-induced motion. It reads stacks of movies that are stored in MRC/CCP4 format.
Unblur generates frame sums that can be used in subsequent image processing steps and optionally applies an exposure-dependent filter to maximize the signal at all resolutions in the frame averages.
Movie frame sums can also be calculated using Summovie, which uses the alignment results from a prior run of Unblur. The idea for this procedure was originally developed by Brilot et al. and Campbell
et al [1] [2]. Unblur was written by Timothy Grant and Alexis Rohou and is described in [3]. 1. Brilot, AF, Chen JZ, Cheng A, Pan J, Harrison SC, Potter CS, Carragher B, Henderson R, Grigorieff N.
2012. Beam-induced motion of vitrified specimen on holey carbon film.. J Struct Biol. 177:630-637. 2. Campbell, MG, Cheng A, Brilot AF, Moeller A, Lyumkis D, Veesler D, Pan J, Harrison SC, Potter CS,
Carragher B et al.. 2012. Movies of ice-embedded particles enhance resolution in electron cryo-microscopy. Structure. 20:1823–1828. 3. Grant, T, Grigorieff N. 2015. Measuring the optimal exposure
for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6. eLife. 4(e06980):1-19.
You will need to load all module(s) on any one of the lines below before the "unblur/1.0.2" module is available to load.
GCC/7.3.0-2.30 OpenMPI/3.1.1
Help:
Description
===========
Unblur is used to align the frames of movies recorded on an electron microscope to reduce image blurring due to beam-induced motion. It reads stacks of movies that are stored in MRC/CCP4 format. Unblur generates frame sums that can be used in subsequent image processing steps and optionally applies an exposure-dependent filter to maximize the signal at all resolutions in the frame averages. Movie frame sums can also be calculated using Summovie, which uses the alignment results from a prior run of Unblur. The idea for this procedure was originally developed by Brilot et al. and Campbell et al [1] [2]. Unblur was written by Timothy Grant and Alexis Rohou and is described in [3].
1. Brilot, AF, Chen JZ, Cheng A, Pan J, Harrison SC, Potter CS, Carragher B, Henderson R, Grigorieff N. 2012. Beam-induced motion of vitrified specimen on holey carbon film.. J Struct Biol. 177:630-637.
2. Campbell, MG, Cheng A, Brilot AF, Moeller A, Lyumkis D, Veesler D, Pan J, Harrison SC, Potter CS, Carragher B et al.. 2012. Movies of ice-embedded particles enhance resolution in electron cryo-microscopy. Structure. 20:1823–1828.
3. Grant, T, Grigorieff N. 2015. Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6. eLife. 4(e06980):1-19.
More information
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- Homepage: https://www.cl.cam.ac.uk/~mgk25/jbigkit/
Best,
HPC team